ModuloStat: An Internet of Things’ Path to Continuous Cultures in Mini-Bioreactors

ModuloStat is a modular and open-source framework that facilitates continuous culture in mini-bioreactors. The system is grounded on digital fabrication tools and programmable electronics easily accessible in FabLabs. Maintaining a culture is divided into tasks assigned to dedicated printed circuit boards with a microcontroller connected to a Wi-Fi network. According to Internet of Things principles, each board operates a set of sensors and actuators autonomously and can receive and send information. The boards are stacked to implement complex behaviors and can be modified to accommodate new features. A thermoregulated box holds the components and can be placed on a laboratory bench or transported under a sterile hood for inoculation.

Documentation and sources

To retrieve ModuloStat © project, visit https://forge.inrae.fr/modulostat/modulostat or https://doi.org/10.57745/BPIUT7.
It includes:
- Assembly_instructions on how to build a standard Modulostat system including pictures and list of materials with references and links;
- Documentation of the various components including electric circuits description and programming principles on how to operate them;
- Images used as illustrations;
- Manufacturing files for 3D printing, laser cutting or PCB making;
- Sources for the various models and programs.

Associated publications

ACS Omega 2025.

Licence

ModuloStat © 2019 by Cyprien GUERIN & Pierre NICOLAS is licensed under CC BY-NC-SA 4.0.
To view a copy of this license, visit https://creativecommons.org/licenses/by-nc-sa/4.0/.
More details in the LICENCE file.